Linker histones tune the length and shape of chromosomes
Date:
October 4, 2021
Source:
Rockefeller University
Summary:
A new study finds that proteins known as linker histones control
the complex coiling process that determines whether DNA will
wind into long and thin chromosomes, made up of many small loops,
or short and thick chromosomes with fewer large loops.
FULL STORY ========================================================================== Human life hinges on the ability of our cells to cram six feet of DNA
into a 10-micron nucleus -- equivalent to fitting a mile of string
inside one green pea. But stuffing genes into cramped quarters is only
half the battle. The DNA must also remain organized, carefully coiled
into loops that ensure the information remains readily accessible and
not a tangled mess.
==========================================================================
Now, new research has identified proteins called linker histones as the
factor that controls whether DNA winds into long and thin chromosomes,
made up of many small loops, or short and thick chromosomes with fewer
large loops. The findings, published in eLife, are the first to describe
how chromosome shape is tuned by linker histones at the molecular level.
"The linker histone was once thought to impact only a narrow range of
the genetic material," says Rockefeller's Hironori Funabiki. "We have
now shown that it controls the number of loops in the chromosome and its ultimate shape, a much larger regulation space than expected." Beyond
"beads on a string" Genetic material is organized around a nucleosome --
often depicted as a bead on a string, with a length of DNA "string" wound around a central protein "bead." The string is clamped to its bead by a
sort of protein clip -- the linker histone -- which is also involved in
folding multiple nucleosome beads into chromatin fibers. These fibers
form chromosomes after they are ratcheted through a molecular motor,
the condensin, that organizes chromatin into loops.
Chromosomes come in a wide range of shapes across species and cell types, largely based on the size of each chromatin loop. Funabiki draws an
example from the familiar (and frustrating) experience of coiling wired earphones. If you wrap them into many tiny loops, the headphones will
fit neatly into your pocket. If, however, you wind the wires into only a
few large loops, the earphones form a bulky mass. Similarly, a greater
number of small loops will give rise to longer, thinner chromosomes;
a few large loops of chromatin will form shorter, thicker chromosomes.
========================================================================== Scientists knew that loop formation lay at the heart of chromosome size
and shape, but how various cells tuned this process to form larger or
smaller loops remained a mystery.
A new role for linker histone Funabiki and colleagues set out to solve
this mystery. Using a method developed by Job Dekker at the University
of Massachusetts Medical School, the team analyzed DNA from frog eggs
and found that linker histones -- beyond clamping strings to beads and organizing them into fibers -- also prevent condensin from binding to nucleosomes and forming chromatin loops.
A picture of loop formation began to emerge, with linker histones at
the very heart of the process. Changing the shape of a chromosome, the researchers found, is a simple matter of increasing or decreasing the
amount of linker histone available to inhibit condensin.
When a high concentration of linker histone blocks condensin, the protein complex is able to make fewer loops of chromatin. Since only a handful
of loops are forming, there's enough slack in the line for those loops to develop into large coils that will eventually bunch up into short, thick chromosomes. Lower concentrations of linker histone kick off the opposite process: condensin is free to form many more loops, so there is less
fiber available to contribute to each loop. The result is a large number
of smaller loops, which compress neatly into long, thin chromosomes.
Funabiki speculates that cells may have evolved the ability to tune
chromosome length in order to speed up or slow down their growth. "The
longer the chromosome is, the more time it takes to separate during cell division," he says. "Frog eggs are exposed to dangerous environments,
so speed is important.
Successful reproduction depends on how quickly the eggs can turn into
tadpoles and escape. Perhaps frog eggs maintain shorter chromosomes
to allow for rapid cell division." In the future, Funabiki's lab will
explore whether linker histones play a similar role in influencing the
size and shape of human chromosomes. "This initial work only involved
frog eggs," says lead author Pavan S. Choppakatla, a member of the
Funabiki lab. "We are now looking at linker histones in human eggs
and somatic cells, to see whether our findings are widely applicable." ========================================================================== Story Source: Materials provided by Rockefeller_University. Note:
Content may be edited for style and length.
========================================================================== Journal Reference:
1. Pavan Choppakatla, Bastiaan Dekker, Erin E Cutts, Alessandro
Vannini, Job
Dekker, Hironori Funabiki. Linker histone H1.8 inhibits
chromatin binding of condensins and DNA topoisomerase II to tune
chromosome length and individualization. eLife, 2021; 10 DOI:
10.7554/eLife.68918 ==========================================================================
Link to news story:
https://www.sciencedaily.com/releases/2021/10/211004153745.htm
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