Oocyte oxidative DNA damage may be involved in minimal/mild endometriosis-related infertility
Authors
Michele G. Da Broi PhD,
Alceu A. Jordão-Jr PhD,
Rui A. Ferriani MD, PhD,
Paula A. Navarro MD, PhD
First published: 17 January 2018
DOI: 10.1002/mrd.22943

Abstract
Early endometriosis is associated with infertility, and oxidative stress may play a role in the pathogenesis of disease-related infertility. This prospective case-control study aimed to compare the presence of oxidative stress markers in the follicular
microenvironment and systemic circulation of infertile women with minimal/mild endometriosis (EI/II) versus individuals undergoing controlled ovarian stimulation for intracytoplasmic sperm injection (ICSI). Seventy-one blood samples (27 from infertile
women with EI/II and 44 controls with tubal and/or male infertility factor) and 51 follicular fluid samples (19 EI/II and 32 controls) were obtained on the day of oocyte retrieval. Total hydroperoxides (FOX1), reduced glutathione, vitamin E, Superoxide
dismutase, total antioxidant capacity, malondialdehyde, advanced oxidation protein products, and 8-hydroxy-2′-deoxyguanosine (8OHdG) concentrations were measured in both fluids. Women with EI/II showed higher FOX1 (8.48 ± 1.72 vs. 7.69 ± 1.71â
€‰Î¼mol/g protein) and lower total antioxidant capacity (0.38 ± 0.18 vs. 0.46 ± 0.15 mEq Trolox/L) concentrations in serum, and higher 8OHdG concentrations (24.21 ± 8.56 vs. 17.22 ± 5.6 ng/ml) in follicular fluid compared with
controls. These data implicate both systemic and follicular oxidative stress may in infertile women with EI/II undergoing controlled ovarian stimulation for ICSI. Furthermore, the elevated 8OHdG concentrations in follicular fluid of women with EI/II may
be related to compromised oocyte quality.

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REVIEW ARTICLE
Is stored iron safe?
JEROME L. SULLIVAN
GAINESVILLE, FLORIDA
Abbreviations: 80HdG 8-hydroxydeoxyguanosine; ROS reactive oxygen species It's not what we don't know about nutrition that hurts us, it is what we know for sure that turns out to be dead wrong.
-attributed to Victor Herbert, MD, JD

https://www.unboundmedicine.com/medline/citation/15614249/Is_stored_iron_safe

Studies of oxidative DNA damage and iron merit mention in this context. 8OHdG has been widely used as a marker of oxidative DNA damage; in insects, its levels have been found to be inversely associated with life expectancy.45 In men and women, the
urinary concentration of 8OHdG was recently shown to increase with the serum ferritin level (Fig 1).46 Dependence of 8OHdG on serum ferritin is seen within the range of conventionally normal ferritin values. The data are compatible with the conclusion
that acquisition of even very small amounts of stored iron in human beings enhances resting levels of oxidative DNA damage. Two studies in rodents have found that iron deprivation is associated with much lower 8OHdG levels and diminished pathologic
endpoints.26,36 The findings of these iron-deprivation experiments suggest that the increase in 8OHdG with serum ferritin in the human data of Nakano et al 46 is not a spurious association but instead is caused by the acquisition of stored iron. Taken
together, these findings challenge the traditional notion that stored iron is safe and support the possibility that iron restriction increases longevity in mammals.
From the Department of Pathology, Immunology, and Laboratory
Medicine, University of Florida College of Medicine.
Submitted for publication July 8, 2004; accepted for publication
October 7, 2004.
Reprint requests: Jerome L. Sullivan, MD, PhD, 315 Salvador
Square, Winter Park, FL 32789; e-mail: jlsul...@pol.net.
J Lab Clin Med 2004;144:280-4.
0022-2143/$
© 2004 Elsevier Inc. All rights reserved.
doi:10.1016/j.lab.2004.10.006
280

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