• Oxidative DNA Damage In Infertility

    From ironjustice@21:1/5 to All on Sat Feb 10 08:23:50 2018
    Oocyte oxidative DNA damage may be involved in minimal/mild endometriosis-related infertility
    Michele G. Da Broi PhD,
    Alceu A. Jordão-Jr PhD,
    Rui A. Ferriani MD, PhD,
    Paula A. Navarro MD, PhD
    First published: 17 January 2018
    DOI: 10.1002/mrd.22943

    Early endometriosis is associated with infertility, and oxidative stress may play a role in the pathogenesis of disease-related infertility. This prospective case-control study aimed to compare the presence of oxidative stress markers in the follicular
    microenvironment and systemic circulation of infertile women with minimal/mild endometriosis (EI/II) versus individuals undergoing controlled ovarian stimulation for intracytoplasmic sperm injection (ICSI). Seventy-one blood samples (27 from infertile
    women with EI/II and 44 controls with tubal and/or male infertility factor) and 51 follicular fluid samples (19 EI/II and 32 controls) were obtained on the day of oocyte retrieval. Total hydroperoxides (FOX1), reduced glutathione, vitamin E, Superoxide
    dismutase, total antioxidant capacity, malondialdehyde, advanced oxidation protein products, and 8-hydroxy-2′-deoxyguanosine (8OHdG) concentrations were measured in both fluids. Women with EI/II showed higher FOX1 (8.48 ± 1.72 vs. 7.69 ± 1.71
    μmol/g protein) and lower total antioxidant capacity (0.38 ± 0.18 vs. 0.46 ± 0.15 mEq Trolox/L) concentrations in serum, and higher 8OHdG concentrations (24.21 ± 8.56 vs. 17.22 ± 5.6 ng/ml) in follicular fluid compared with
    controls. These data implicate both systemic and follicular oxidative stress may in infertile women with EI/II undergoing controlled ovarian stimulation for ICSI. Furthermore, the elevated 8OHdG concentrations in follicular fluid of women with EI/II may
    be related to compromised oocyte quality.


    Is stored iron safe?
    Abbreviations: 80HdG 8-hydroxydeoxyguanosine; ROS reactive oxygen species It's not what we don't know about nutrition that hurts us, it is what we know for sure that turns out to be dead wrong.
    -attributed to Victor Herbert, MD, JD


    Studies of oxidative DNA damage and iron merit mention in this context. 8OHdG has been widely used as a marker of oxidative DNA damage; in insects, its levels have been found to be inversely associated with life expectancy.45 In men and women, the
    urinary concentration of 8OHdG was recently shown to increase with the serum ferritin level (Fig 1).46 Dependence of 8OHdG on serum ferritin is seen within the range of conventionally normal ferritin values. The data are compatible with the conclusion
    that acquisition of even very small amounts of stored iron in human beings enhances resting levels of oxidative DNA damage. Two studies in rodents have found that iron deprivation is associated with much lower 8OHdG levels and diminished pathologic
    endpoints.26,36 The findings of these iron-deprivation experiments suggest that the increase in 8OHdG with serum ferritin in the human data of Nakano et al 46 is not a spurious association but instead is caused by the acquisition of stored iron. Taken
    together, these findings challenge the traditional notion that stored iron is safe and support the possibility that iron restriction increases longevity in mammals.
    From the Department of Pathology, Immunology, and Laboratory
    Medicine, University of Florida College of Medicine.
    Submitted for publication July 8, 2004; accepted for publication
    October 7, 2004.
    Reprint requests: Jerome L. Sullivan, MD, PhD, 315 Salvador
    Square, Winter Park, FL 32789; e-mail: jlsul...@pol.net.
    J Lab Clin Med 2004;144:280-4.
    © 2004 Elsevier Inc. All rights reserved.

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