Making genetically enhanced sperm better, wikipedia identifies a middle piece where the tail is surrounded by mitochondria, double or half that could variously make them able to swim faster or use less food to stay fertile longer during storage like
encapsulation or better (nuvaring)
tail dip jig is only osmotic area to puff up with food

Other sperm foods at the medium or storage medium could be acetyl-CoA, “CoA is acetylated to acetyl-CoA by the breakdown of carbohydrates through glycolysis and by the breakdown of fatty acids through β-oxidation. Acetyl-CoA then enters the citric
acid cycle, where the acetyl group is oxidized to carbon dioxide and water, and the energy released is captured in the form of 11 ATP and one GTP per acetyl group.”
There is just a possibility that becuase the sperm mitochondria have TCA cycle and can process lipids, that a lipid enriched sperm has lots of food energy ( 8 cal per gram compared with 4), “At low glucose levels, the production of acetyl-CoA is linked
to β-oxidation of fatty acids”

Taking human tissue homogenate, put it in supportive medium and see which human tissues have the longest living mitochondria, those mitochondrial types could be instantiated at sperm to generate longer sperm life and greater motility; also compare
cytosol and organelle function at longest lived tissues and noting some human tissues revive after lyophilization https://www.freepatentsonline.com/y2018/0104282.html the human tissue cytes that revive after lyophilization are a guide to the organelles,
their particular size, chemistry that permit successful revival from hydration from lyophiliization

The patent describes successful tissue (skin) lyophilization media with >70% revival that they could test this media on sperm, this may work now:Lyoprotectant

Solution
Composition Cell Viability Comments

10% DMSO, 12.5% <50% viable cells Not selected
HSA in D-PBS
25% HSA in D-PBS No viable cells Not selected
0.25M Trehalose, Majority of cells are Not selected
12.5% HSA in D- viable
PBS
0.1M Trehalose in No viable cells Not selected
D-PBS
0.25M Trehalose in Majority of cells are Inconsistent results
D-PBS viable
0.5M Trehalose in Majority of cells are Selected: highest cell
D-PBS viable viability by qualitative
assessment with simplest
composition

1M Trehalose in D- Majority of cells are Similar results to 0.5M
PBS viable trehalose.
0.25M Trehalose, Majority of cells are Not selected, causes brown
1 mg/mL Catechin in viable coloration of amnion
D-PBS

“samples from this experiment were tested for cell viability, and VLAM should have >70% viable cells for the rinse to deem acceptable.”

A technology that could raise the viability of lyophilized genetically enhanced sperm is coculturing, or just mixing, then 24 hours of stabilization, dermatocytes with sperm cytes such that the proportions keep the sperm from ever overlapping, the cell-
to-cell contact surface may be particularly benign, and along with regular dermatocytes if seminal vesicle membrane tissues lyophilize and revive well then that could be tissue cultured to be used as the mix-with-sperm possible lyophilization preservative

Lyophilizing freeze dries sperm, but what of partial lyohilization, frozen thawed sperm is fertile, so at a range of 100 samples, each at 1%, 2%, 3%…100% dewatered from partial freeze drying spread out as a monolayer powder on a porous bottom (polymer)
sheet, which of the 1-100 percentage steps survive, after freezing revive? And, what is the foil package under nitrogen survival or microencapsulation survival of each stepwise 1%? At microencapsulation 100% of fresh sperm survive 1 month (published)
There is an aqueous osmotically neutral way to have a 50% water, 50% some other thing that has very gradual osmotic change (maybe its just stirring and titration) such that the cyte omits shriveling or bursting, The some other thing might be fructose or
a sperm edible electrically neutral amino acid; What is the fresh sperm, lowest percentage of cyte body water that can support a moving vital the moving vital sperm number, Then find out which least water % prep environment permits the revival of those
no-longer-swimming -sperm? Those numbers suggest a possible number for a fractional lyophilization, high revival after storage at consumer packaging genetically enhanced sperm product ; If fresh sperm still swim at 70% less body water, that means
something, if sperm that have ceased swimming on their own at 80% body water can be revived to swim starting at 50-100% body water that is something

It is pretty likely 99% hydrated is fine, so what about if 50% hydrated stays functional for many months, is a humid gel microbead powder, that can go in a cervical ring (nuvaring) or break n shake vaginal applicator

Freeze drying the already dehydrated, but still vital sperm could also work better as the interval of freezing is less: (If fresh sperm still swim at 70% less internal body water, that means something, if sperm that have ceased swimming on their own at
80% internal body water can be revived to swim starting at 50-100% internal body water that is something) according to something like that you could lyophilize the 70% osmotically dehydrated sperm with just 30% of the shock-to-sperm interval

Thaw omostic internal organelle “shock”; when it rehydrates with water, but the 1st 10% of rehydration comes with a very high organelle ion difference so it does anything from physically popping them or shriveling them, to wiping out their chemistry.
pH could change an order of magnitude (1 pH number) just from having the first minute of rehydration dissolve the cytoplasm’s ions; As a lyophilization survival strategy preload sperm with ion balancers like more K(potassium) without inorganic ion so
like Kaminoacid, or even K-ATP, if it the other way then more chloride, (Aminoacid-cl?) (MgCl2?) calcium chloride?; Perhaps if cyte is naturally acidic, bicoarbonate ion like Kco3 or even amino acidCO3 or MgOH would balance and buffer pH throughout
entire 1-100% of hydration change

Also, a 1-100% (100) rehydration steps-maintained buffer and ratio maintener is new to me, as a“stepwise no-shock buffer;

Other buffer chemicals for osmotic shock, or also misaligned anion and cation ratios from stepwise hydration: if cyte is naturally basic, buffer the 1-100% cytoplasm hydration steps (10-100x ion concentration during the first 2 seconds after pouring or
shaking with water/plasma replacer) Treat basicity with citric acid (TCA cycle), Malic Acid (TCA cycle), Acetic acid (also a food for some cytes); Perhaps if cyte is naturally acidic, bicoarbonate ion like Kco3 or even amino acidCO3 or MgOH would balance
and buffer pH throughout entire 1-100% of hydration change
If sperm as part of their functionality produced their own buffer (acid+salt, others) chemicals, then they might revive better after lyophilization

So, at a break n shake lightstick-like lyophilized sperm reconsituter/rehydrator the velocity at which the water reaches the interior of the sperm cytes might determine the amount of mess-up to the cyte so rehydrating fully as rapidly as possible might
cause greater reconstituted genetically enhanced sperm vitality, one way to do that could be nonharmful sonic vibration. The Break n Shake genetically enhanced sperm stick vaginal insert could have a button battery and a specially tuned piezoelectric
buzzer for 2 cents (alibaba; piezo buzzer with wires 1 cent, battery .6 cent), the tuned sonication would make the regularization of cyte pH, ion concentrations, and other things return to those of fesh sperm much more rapidly than just the break n shake
n wait 3 minutes approach. Being able to use the sperm in less than three minutes is beneficial for convenience, and adding a color change LED (from blue to green) (green means go) to the break n shake stick that already has a battery and piezo element
is likely less than 1 cent

buzzer(pH and ion gradients 100 times larger than usual concentrations, totally different chemical ratios during redissolving of cytoplasm and plumping up organelles phase)
piezoelectric get water in the organelles rapidly from vibration button cell (alibaba .6 cents)


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All technologies, ideas, and inventions of Treon Sebastian Verdery are public domain at JUly 8,2023AD and previously, as well as after that date

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