• ugly

    From John Larkin@21:1/5 to All on Wed Aug 7 09:01:27 2024
  • From Phil Hobbs@21:1/5 to John Larkin on Wed Aug 7 16:22:05 2024
    John Larkin <jjlarkin@highlandtechnology.com> wrote:

    https://www.planetanalog.com/wp-content/uploads/Fig-1-STED-precise-temp-measurement.png?resize=1260,709



    Brilliant soldering job, for sure. :(

    Cheers

    Phil Hobbs

    --
    Dr Philip C D Hobbs Principal Consultant ElectroOptical Innovations LLC / Hobbs ElectroOptics Optics, Electro-optics, Photonics, Analog Electronics

    --- SoupGate-Win32 v1.05
    * Origin: fsxNet Usenet Gateway (21:1/5)
  • From John Larkin@21:1/5 to pcdhSpamMeSenseless@electrooptical. on Wed Aug 7 10:25:58 2024
    On Wed, 7 Aug 2024 16:22:05 -0000 (UTC), Phil Hobbs <pcdhSpamMeSenseless@electrooptical.net> wrote:

    John Larkin <jjlarkin@highlandtechnology.com> wrote:

    https://www.planetanalog.com/wp-content/uploads/Fig-1-STED-precise-temp-measurement.png?resize=1260,709



    Brilliant soldering job, for sure. :(

    Cheers

    Phil Hobbs

    I like the fingerprints on the Scotch tape, and the hairball on the
    probe. And all the dust.

    --- SoupGate-Win32 v1.05
    * Origin: fsxNet Usenet Gateway (21:1/5)
  • From Bill Sloman@21:1/5 to John Larkin on Thu Aug 8 16:46:35 2024
    On 8/08/2024 3:25 am, John Larkin wrote:
    On Wed, 7 Aug 2024 16:22:05 -0000 (UTC), Phil Hobbs <pcdhSpamMeSenseless@electrooptical.net> wrote:

    John Larkin <jjlarkin@highlandtechnology.com> wrote:

    https://www.planetanalog.com/wp-content/uploads/Fig-1-STED-precise-temp-measurement.png?resize=1260,709



    Brilliant soldering job, for sure. :(

    Cheers

    Phil Hobbs

    I like the fingerprints on the Scotch tape, and the hairball on the
    probe. And all the dust.

    None of which matters.

    Stimulate emission depletion (STED) microscopy is not a mode of
    temperature measurement that I had heard of, and John Larkin is likely
    to be just as ignorant, but rather than finding out what is actually
    going on he merely sneers about the unpretentious image.

    Google managed to find this for me.

    "Stimulated emission depletion (STED) microscopy delivers resolutions
    better than 20 nm. Due to significant progress in STED instrumentation
    and the commercialization of new, photo-stable dyes, STED is now widely accessible. However, although STED offers unlimited resolution in
    theory, photobleaching comes into play in practice. Mediating its
    effects can require tuning down the resolution or recording fewer time
    steps and smaller 3D-volumes than desired. Besides Adaptive
    Illumination, a solution to circumvent the physical limitations of
    current labeling technology is the adaptation of the PAINT-concept
    (point accumulation for imaging in nanoscale topography) to STED
    microscopy. PAINT is based on the application of exchangeable labels
    that only temporarily bind to their target structures. During
    acquisition, these labels are constantly replenished from a large pool
    in the imaging medium, providing stable sample brightness even at the
    highest resolutions.

    The resolution of a STED microscope scales with the intensity of the
    STED laser. Unfortunately, an increase in resolution is therefore often connected to an increase in photo-bleaching. The available fluorescence
    photon budget thus can hinder time lapses as well as volume imaging.
    This is because with time lapse imaging, the sample area is illuminated multiple times and the number of fluorophores is concomitantly reduced
    each time. Similarly, when imaging volumes, adjacent z-planes are imaged
    in succession, so that each plane is subjected to multiple rounds of illumination and therefore photobleached to a certain extent each time.
    As a result, microscopists often need to sacrifice either resolution,
    the number of time steps or the number of z-planes recorded in order to
    reduce photobleaching to an acceptable level.

    The situation described above was particularly true for early
    implementations of STED microscopy, when very strong sample illumination
    met a small number of photo-stable fluorophores. Today, major
    developments in STED instrumentation, such as the use of optimized
    pulsed STED lasers 1, highly efficient detection via APDs, and Adaptive Illumination 2, 3, 4 now allow a strong reduction of sample
    illumination. Currently, state-of-the-art STED microscopes readily offer resolutions reaching <20 nm and allow the acquisition of live cell
    movies and image stacks. At the same time, a vast range of optimized,
    bright, and photostable fluorophores for STED imaging is now
    commercially available and comes with many conjugation chemistries for
    diverse applications 5-9.

    Nevertheless, the achievable resolution and number of image frames are
    still ultimately limited by photobleaching, albeit at a much higher
    level than only a few years ago.

    On the other hand, PAINT allows to suppress or bypass the photobleaching process and therefore offers additional resolution and extended
    time-lapse or volume imaging. With conventional PAINT microscopy,
    individual, transiently binding dyes are located precisely using single molecule localization microscopy 10, 11. Most importantly, fluorophores
    are constantly exchanged: bleached fluorophores are replaced with fresh
    ones from the large reservoir of the imaging medium using labels with
    transient binding properties. This allows for fast and continuous
    exchange of fluorophores during image acquisition.

    Recently, Spahn et al. 12 have successfully adopted exchangeable
    fluorophores for use with STED microscopy. In contrast to classical
    PAINT, which requires very few dyes bound at a single time, staining for
    STED was optimized to provide high densities of bound dyes while
    allowing fast replenishment from the imaging medium. Interestingly, this
    can be implemented with a large range of staining techniques, including
    simple lipophilic dyes, DNA stains, toxin- and peptide-conjugated dyes
    as well as with immunostaining with DNA-labeled antibodies."

    Bizarre stuff, but no doubt fascinating to some.

    --
    Bill Sloman, Sydney





    --
    This email has been checked for viruses by Norton antivirus software. www.norton.com

    --- SoupGate-Win32 v1.05
    * Origin: fsxNet Usenet Gateway (21:1/5)
  • From Jeff Layman@21:1/5 to Bill Sloman on Thu Aug 8 08:25:09 2024
    On 08/08/2024 07:46, Bill Sloman wrote:
    On 8/08/2024 3:25 am, John Larkin wrote:
    On Wed, 7 Aug 2024 16:22:05 -0000 (UTC), Phil Hobbs
    <pcdhSpamMeSenseless@electrooptical.net> wrote:

    John Larkin <jjlarkin@highlandtechnology.com> wrote:

    https://www.planetanalog.com/wp-content/uploads/Fig-1-STED-precise-temp-measurement.png?resize=1260,709



    Brilliant soldering job, for sure. :(

    Cheers

    Phil Hobbs

    I like the fingerprints on the Scotch tape, and the hairball on the
    probe. And all the dust.

    None of which matters.

    Stimulate emission depletion (STED) microscopy is not a mode of
    temperature measurement that I had heard of, and John Larkin is likely
    to be just as ignorant, but rather than finding out what is actually
    going on he merely sneers about the unpretentious image.

    Google managed to find this for me.

    (snip)

    If you'd just gone to the parent webpage (which is usually worth trying)
    at <https://www.planetanalog.com> you'd have found the article mentioned
    that the photo came from. That was at <https://www.planetanalog.com/new-test-approach-yields-ultra-focused-temperature-measurements/>.
    That provides a link to the original article at <https://www.science.org/doi/10.1126/sciadv.ado6268>. Strangely, that
    page does not have the photo, nor does the pdf link at the end of the
    page to supplementary material.

    --
    Jeff

    --- SoupGate-Win32 v1.05
    * Origin: fsxNet Usenet Gateway (21:1/5)
  • From Phil Hobbs@21:1/5 to legg on Thu Aug 8 15:10:52 2024
    legg <legg@nospam.magma.ca> wrote:
    On Wed, 7 Aug 2024 16:22:05 -0000 (UTC), Phil Hobbs <pcdhSpamMeSenseless@electrooptical.net> wrote:

    John Larkin <jjlarkin@highlandtechnology.com> wrote:

    https://www.planetanalog.com/wp-content/uploads/Fig-1-STED-precise-temp-measurement.png?resize=1260,709



    Brilliant soldering job, for sure. :(

    Cheers

    Phil Hobbs

    You can certainly tell where the robots left off and the humins
    stepped in . . . .

    RL


    And then the monkeys.

    Cheers

    Phil Hobbs

    --
    Dr Philip C D Hobbs Principal Consultant ElectroOptical Innovations LLC / Hobbs ElectroOptics Optics, Electro-optics, Photonics, Analog Electronics

    --- SoupGate-Win32 v1.05
    * Origin: fsxNet Usenet Gateway (21:1/5)
  • From legg@21:1/5 to pcdhSpamMeSenseless@electrooptical. on Thu Aug 8 10:49:32 2024
    On Wed, 7 Aug 2024 16:22:05 -0000 (UTC), Phil Hobbs <pcdhSpamMeSenseless@electrooptical.net> wrote:

    John Larkin <jjlarkin@highlandtechnology.com> wrote:

    https://www.planetanalog.com/wp-content/uploads/Fig-1-STED-precise-temp-measurement.png?resize=1260,709



    Brilliant soldering job, for sure. :(

    Cheers

    Phil Hobbs

    You can certainly tell where the robots left off and the humins
    stepped in . . . .

    RL

    --- SoupGate-Win32 v1.05
    * Origin: fsxNet Usenet Gateway (21:1/5)
  • From John Larkin@21:1/5 to All on Thu Aug 8 13:34:40 2024
    On Thu, 8 Aug 2024 08:25:09 +0100, Jeff Layman <Jeff@invalid.invalid>
    wrote:

    On 08/08/2024 07:46, Bill Sloman wrote:
    On 8/08/2024 3:25 am, John Larkin wrote:
    On Wed, 7 Aug 2024 16:22:05 -0000 (UTC), Phil Hobbs
    <pcdhSpamMeSenseless@electrooptical.net> wrote:

    John Larkin <jjlarkin@highlandtechnology.com> wrote:

    https://www.planetanalog.com/wp-content/uploads/Fig-1-STED-precise-temp-measurement.png?resize=1260,709



    Brilliant soldering job, for sure. :(

    Cheers

    Phil Hobbs

    I like the fingerprints on the Scotch tape, and the hairball on the
    probe. And all the dust.

    None of which matters.

    Stimulate emission depletion (STED) microscopy is not a mode of
    temperature measurement that I had heard of, and John Larkin is likely
    to be just as ignorant, but rather than finding out what is actually
    going on he merely sneers about the unpretentious image.

    Google managed to find this for me.

    (snip)

    If you'd just gone to the parent webpage (which is usually worth trying)
    at <https://www.planetanalog.com> you'd have found the article mentioned
    that the photo came from. That was at ><https://www.planetanalog.com/new-test-approach-yields-ultra-focused-temperature-measurements/>.
    That provides a link to the original article at ><https://www.science.org/doi/10.1126/sciadv.ado6268>. Strangely, that
    page does not have the photo, nor does the pdf link at the end of the
    page to supplementary material.

    The photo is probably random decorative clip art that has nothing to
    do with the article.

    It appears to be a wire bonding experiment.

    --- SoupGate-Win32 v1.05
    * Origin: fsxNet Usenet Gateway (21:1/5)
  • From Bill Sloman@21:1/5 to John Larkin on Sat Aug 10 01:43:41 2024
    On 9/08/2024 6:34 am, John Larkin wrote:
    On Thu, 8 Aug 2024 08:25:09 +0100, Jeff Layman <Jeff@invalid.invalid>
    wrote:

    On 08/08/2024 07:46, Bill Sloman wrote:
    On 8/08/2024 3:25 am, John Larkin wrote:
    On Wed, 7 Aug 2024 16:22:05 -0000 (UTC), Phil Hobbs
    <pcdhSpamMeSenseless@electrooptical.net> wrote:

    John Larkin <jjlarkin@highlandtechnology.com> wrote:

    https://www.planetanalog.com/wp-content/uploads/Fig-1-STED-precise-temp-measurement.png?resize=1260,709



    Brilliant soldering job, for sure. :(

    Cheers

    Phil Hobbs

    I like the fingerprints on the Scotch tape, and the hairball on the
    probe. And all the dust.

    None of which matters.

    Stimulate emission depletion (STED) microscopy is not a mode of
    temperature measurement that I had heard of, and John Larkin is likely
    to be just as ignorant, but rather than finding out what is actually
    going on he merely sneers about the unpretentious image.

    Google managed to find this for me.

    (snip)

    If you'd just gone to the parent webpage (which is usually worth trying)
    at <https://www.planetanalog.com> you'd have found the article mentioned
    that the photo came from. That was at
    <https://www.planetanalog.com/new-test-approach-yields-ultra-focused-temperature-measurements/>.
    That provides a link to the original article at
    <https://www.science.org/doi/10.1126/sciadv.ado6268>. Strangely, that
    page does not have the photo, nor does the pdf link at the end of the
    page to supplementary material.

    The photo is probably random decorative clip art that has nothing to
    do with the article.

    It appears to be a wire bonding experiment.

    Jeff Layman found the original article from which you'd snipped the
    photograph, but you still don't seem to have read it.

    The photograph doesn't seem to have much to do with a custom scanning
    confocal microscope with a 10mm working distance, but the optics might
    be buried in the metal bar at the top of the image. One of my friends
    invented and patented an improved scanning confocal microscope, and
    made a couple of million dollars out of the invention, and - in theory -
    I could ask him about it.

    Since you clearly aren't interested in getting better informed, I'm not
    going to bother.

    --
    Bill Sloman, Sydney





    --
    This email has been checked for viruses by Norton antivirus software. www.norton.com

    --- SoupGate-Win32 v1.05
    * Origin: fsxNet Usenet Gateway (21:1/5)
  • From Lasse Langwadt@21:1/5 to Phil Hobbs on Mon Aug 12 21:49:06 2024
    On 8/7/24 18:22, Phil Hobbs wrote:
    John Larkin <jjlarkin@highlandtechnology.com> wrote:

    https://www.planetanalog.com/wp-content/uploads/Fig-1-STED-precise-temp-measurement.png?resize=1260,709



    Brilliant soldering job, for sure. :(


    looks pretty good if it was done with a rusty nail heated with a candle

    --- SoupGate-Win32 v1.05
    * Origin: fsxNet Usenet Gateway (21:1/5)
  • From legg@21:1/5 to All on Tue Aug 13 09:27:26 2024
    On Mon, 12 Aug 2024 21:49:06 +0200, Lasse Langwadt <llc@fonz.dk>
    wrote:

    On 8/7/24 18:22, Phil Hobbs wrote:
    John Larkin <jjlarkin@highlandtechnology.com> wrote:

    https://www.planetanalog.com/wp-content/uploads/Fig-1-STED-precise-temp-measurement.png?resize=1260,709



    Brilliant soldering job, for sure. :(


    looks pretty good if it was done with a rusty nail heated with a candle

    lead-free solder . . ..

    RL

    --- SoupGate-Win32 v1.05
    * Origin: fsxNet Usenet Gateway (21:1/5)
  • From Lasse Langwadt@21:1/5 to legg on Tue Aug 13 17:10:55 2024
    On 8/13/24 15:27, legg wrote:
    On Mon, 12 Aug 2024 21:49:06 +0200, Lasse Langwadt <llc@fonz.dk>
    wrote:

    On 8/7/24 18:22, Phil Hobbs wrote:
    John Larkin <jjlarkin@highlandtechnology.com> wrote:

    https://www.planetanalog.com/wp-content/uploads/Fig-1-STED-precise-temp-measurement.png?resize=1260,709



    Brilliant soldering job, for sure. :(


    looks pretty good if it was done with a rusty nail heated with a candle

    lead-free solder . . ..

    can't blame that on lead-free, lead-free solders just fine once you get
    used to it

    --- SoupGate-Win32 v1.05
    * Origin: fsxNet Usenet Gateway (21:1/5)