I'm working on detecting a particular metal ion using fluorescent
dyes in conjunction with absorbance and emission spectroscopy, and I
need to test the chosen dye over a range of pH values (i.e. 10-14). I
first create the dye solution in a beaker and adjust its pH using
NaOH (lets say one dye solution at 12.5 and one at 13.0), then I add
1.9 mL of this to a cuvette along with 0.1 mL of a metal ion in acid solution. However, the cuvette's pH always drops to the same value
(typically 12.3) once I add my 0.1 mL metal ion in acid solution. I'm assuming this is because pH is logarithmic, to reiterate the
proportions are 1.9 mL of the dye solution to 0.1 mL of the metal ion
in acid solution.
Any suggestions on resolving this? I cannot titrate a solution in a
small, 2.5 mL cuvette. I've considered using a buffer from what I've
read but I do not know how to select one, or if they affect absorbance/emission data.
I do not have a background in chemistry but I've been on this project
and am now hitting a brick wall. Any advice or guidance is much
appreciated.
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