EurekAlert!
PUBLIC RELEASE: 10-MAR-2016

Patterns of brain swelling may explain susceptibility of children to cerebral malaria
PLOS


IMAGE
IMAGE: THIS IS A COLOR-CODED T2-WEIGHTED SAGITTAL IMAGE OF AN ECM MOUSE WITH EDEMA ALONG THE OB+RMS. view more

CREDIT: HOFFMAN A, ET AL.

Brain swelling is a strong predictor of death in children with cerebral malaria (a severe form of the disease where parasites have accumulated in brain vessels), and also in mice with experimental cerebral malaria (ECM). A high-resolution whole brain
imaging analysis of swelling in ECM published on March 10th in PLOS Pathogens suggests that cerebral malaria depends on the permissive environment in a specific brain area. The area, which changes during development, functions as a traffic route for
immature nerve cells and immune cells especially in young animals, and allows progression of a strong host immune response into the central nervous system.

Angelika Hoffmann, from University of Heidelberg, Germany, and colleagues monitored the evolution of ECM-associated fluid increase in the brain (called edema, a condition that ultimately leads to brain swelling) in live mice using high-field magnetic
resonance imaging (MRI) with whole-brain coverage. Following 10 malaria-infected mice over time, the researchers saw a distinct pattern of cerebral disease spread. Based on the timing of the earliest symptoms they observed, they added five more mice and
focused specifically on the initial phase of brain abnormalities.

The researchers found that leakiness of the blood-brain-barrier and consecutive edema starts in an area called the olfactory bulb (OB) that is involved in the sense of smell. Before the mice show any obvious disease symptoms, the edema spreads deeper
into the brain along a specific path called the rostral migratory stream (RMS) that is used as a traffic route by immature nerve cells and immune cells. In mice with severe disease, the edema eventually reaches the brain stem, and by that time the
animals start to fall into a comatose state.

The researchers conclude that their findings "provide strong evidence that the OB+RMS, which represents a structurally and functionally unique axis of immune and neuroblast trafficking, is the structure permitting and transmitting the inflammatory
response of ECM deeper into the brain".

Brain imaging results in human malaria patients published by other scientists suggest that swelling starts and spreads along a similar path, that is also known to be a migratory route for nerve-cell precursors (a so-called neurogenic niche) in humans (
with age, nerve cell generation and migration declines). Based on these human studies and their own results in mice, the researchers suggest that "the spread of inflammation in cerebral malaria may be facilitated by a distinct organization of the
neurogenic niche in the young, providing a permissive environment not only for neuronal migration, but also for inflammation".

###

Please use this URL to provide readers access to the paper (Link goes live upon article publication): http://dx.plos.org/10.1371/journal.ppat.1005470

Authors and Affiliations:

Angelika Hoffmann, Felix T. Kurz, Martin Bendszus, Mirko Pham Department of Neuroradiology, Heidelberg University Hospital, Heidelberg, Germany

Angelika Hoffmann, Felix T. Kurz, Sabine Heiland, Xavier Helluy Division of Experimental Radiology, Department of Neuroradiology, Heidelberg University Hospital, Heidelberg, Germany

Johannes Pfeil, Ann-Kristin Mueller Centre for Infectious Diseases, Parasitology Unit, Heidelberg University Hospital, Heidelberg, Germany

Johannes Pfeil Center for Childhood and Adolescent Medicine, General Pediatrics, University Hospital, Heidelberg, Heidelberg, Germany

Johannes Pfeil, Ann-Kristin Mueller German Centre for Infection Research (DZIF), Heidelberg, Germany

Julieta Alfonso, Hannah Monyer Department of Clinical Neurobiology, Medical Faculty of Heidelberg University and German Cancer Research Center (DKFZ), Heidelberg, Germany

Felix Sahm Department of Neuropathology, Heidelberg University Hospital, Heidelberg, Germany; German Cancer Consortium (DKTK), Clinical Cooperation Unit Neuropathology, German Cancer Research Center (DKFZ), Heidelberg, Germany

Xavier Helluy Experimental Physics V, University of Würzburg, Würzburg, Germany

Please contact plospathogens@plos.org if you would like more information.

Funding: This work was supported by an award from the German Society of Pediatric Infectious Diseases (Deutsche Gesellschaft fuer Paediatrische Infektiologie, DGPI) to JP. AH, FTK and FS receive funding from a postdoctoral stipend of the Medical Faculty
of the University of Heidelberg. JP received funding from the German Centre for Infection Research (Deutsches Zentrum fuer Infektionsforschung, DZIF) (grant no. 80006013). MP is supported by a memorial stipend from the Else-Kröner-Fresenius-Foundation.
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing Interests: The authors have declared that no competing interests exist.

Citation: Hoffmann A, Pfeil J, Alfonso J, Kurz FT, Sahm F, Heiland S, et al. (2016) Experimental Cerebral Malaria Spreads along the Rostral Migratory Stream. PLoS Pathog 12(3): e1005470. doi:10.1371/journal.ppat.1005470

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.

Media Contact

Angelika Hoffmann
angelika.hoffmann@med.uni-heidelberg.de
http://www.plos.org

More on this News Release

Patterns of brain swelling may explain susceptibility of children to cerebral malaria
PLOS
JOURNAL
PLOS Pathogens
KEYWORDS

BIOLOGYDISEASE IN THE DEVELOPING WORLDINFECTIOUS/EMERGING DISEASESMEDICINE/HEALTHMICROBIOLOGYNEUROBIOLOGYNEUROCHEMISTRYPARASITOLOGYPHYSIOLOGYPHYSIOLOGY
MULTIMEDIA

Patterns of Brain Swelling May Explain Susceptibility of Children to Cerebral Malaria
Patterns of Brain Swelling May Explain Susceptibility of Children to Cerebral Malaria
(IMAGE)
RELATED JOURNAL ARTICLE

http://dx.doi.org/10.1371/journal.ppat.1005470


http://www.eurekalert.org/pub_releases/2016-03/p-pob030316.php

--- SoupGate-Win32 v1.05
* Origin: fsxNet Usenet Gateway (21:1/5)