Researchers discover drug-resistant environmental mold is capable of
infecting people

Date:
April 25, 2022
Source:
Imperial College London
Summary:
A new study finds that drug-resistant mold is spreading from the
environment and infecting susceptible people's lungs.



FULL STORY ==========================================================================
A new study led by Imperial College London finds that drug-resistant
mould is spreading from the environment and infecting susceptible
people's lungs.


==========================================================================
The researchers found six cases of people infected with a drug-resistant
form of a fungi called Aspergillus fumigatus that could be traced back
to spores in the environment. Their findings use samples from England,
Wales, Scotland and Ireland, and are published in Nature Microbiology.

Aspergillus fumigatus is an environmental mould that can cause fungal
lung disease. While people with healthy lungs clear inhaled spores,
people with lung conditions or weakened immune systems sometimes cannot, meaning the spores may remain in the lungs causing an infection called aspergillosis. Aspergillosis affects 10-20 million people worldwide. The infection is usually treated with an antifungal drug but emerging
resistance to these drugs has been reported.

This resistance has evolved because of the widespread agricultural use
of azole fungicides, the researchers say. Azole drugs that work in a
similar way are the first-line treatment for patients infected with the Aspergillus fumigatus mould, so the fungus' exposure to azole fungicides
in the environment means it is often in a drug-resistant form even before
it even encounters the people it infects.

The researchers call for greater surveillance of Aspergillus fumigatus
in the environment and the clinic to help understand the risk it poses.

Senior author, Professor Matthew Fisher, from Imperial's School
of Public Health, says: "Understanding the environmental hotspots
and genetic basis of evolving fungal drug resistance needs urgent
attention, because resistance is compromising our ability to prevent
and treat this disease." He adds: "The prevalence of drug-resistant aspergillosis has grown from negligible levels before 1999 to up to 3-40%
of cases now across Europe. At the same time, more and more people might
be susceptible to Aspergillus fumigatus infection because of growing
numbers of people receiving stem cell or solid organ transplants, being
on immunosuppressive therapy, or having lung conditions or severe viral respiratory infections."


==========================================================================
In the study, the researchers isolated 218 samples of Aspergillus
fumigatus from across the England, Wales, Scotland and Ireland between
2005 and 2017.

Around 7 in 10 samples were from people infected (153 samples from
143 patients in five hospitals) and the rest were from the environment
(65 samples) - - including from soil, compost, plant bulbs, the air and
other sources.

The researchers extracted the DNA from the sample moulds and sequenced
it to determine whether there was crossover in the resistant spores
found in the environment and in some patients.

They found six strains of Aspergillus fumigatus that existed in the
environment which had infected six patients. The researchers say that
the genetic similarities indicated that the fungus had spread from the environment to the patient.

Lead author, Dr Johanna Rhodes, from Imperial's MRC Centre for Global
Disease Analysis within the School of Public Health, says: "Increasingly,
the cases of aspergillosis seen in the clinic are resistant to first-line
azole drugs.

However, we've not been sure how patients are acquiring these infections -
- whether they develop in the lungs during treatment for the infection,
or whether the mould spores that infect them are drug-resistant in
the first place. Our study finds that both routes of infection are
possible and confirms concerns that pre-resistant mould spores in
the environment are able to enter and infect people's lungs causing harder-to-treat disease." Of the 218 samples, almost half (106 samples)
were resistant to at least one of the first-line azole drugs used in the clinic. Specifically, 48% (104 samples) were resistant to itraconazole,
29% (64 samples) to voriconazole, and 21% to posaconazole. More than 10%
of samples (26, including 23 environmental samples and 3 from patients)
were resistant to two or more azole drugs.



========================================================================== Within the 218 samples, the researchers found 50 new genes associated
with drug resistance. Looking deeper into the DNA, they also found five
new combinations of single digit changes in the DNA (called single
nucleotide polymorphisms, or SNPs) that were associated with drug
resistance, including one which was resistant to multiple drugs.

Looking at the genes of the Aspergillus fumigatus samples, the researchers found that there were two distinct groups -- group A (123 of the 218
samples) and group B (95 samples). Around 80% of samples in group A
were drug resistant, while about 85% of group B were not. While the two
groups were distinct, the researchers found signs of them swapping genetic material, and in some cases creating new versions of drug resistance.

The researchers say these findings indicate that the full spectrum of
azole- resistance in Aspergillus fumigatus is yet to be understood.

Dr Rhodes says: "Our findings highlight new ways of resistant infections passing to people and show that a better understanding of where and
how Aspergillus fumigatus is generating drug resistance is needed."
The study was funded by the Natural Environment Research Council, the
Wellcome Trust, Gilead Sciences and the Medical Research Council. It
involved researchers from Imperial, King's College University Hospital,
Trinity College Dublin, University of Aberdeen, The University of
Manchester, Public Health Wales, Lee Kong Chian School of Medicine,
Nanyang Technological University, Beaumont Hospital, Tallaght University Hospital, University of Nottingham, the National Mycology Reference
Laboratory at the UK Health Security Agency, University of Exeter,
and Leeds Teaching Hospitals National Health Service Trust.


========================================================================== Story Source: Materials provided by Imperial_College_London. Original
written by Emily Head.

Note: Content may be edited for style and length.


========================================================================== Journal Reference:
1. Rhodes, J., Abdolrasouli, A., Dunne, K. et al. Population genomics
confirms acquisition of drug-resistant Aspergillus fumigatus
infection by humans from the environment. Nat Microbiol, 2022 DOI:
10.1038/s41564-022- 01091-2 ==========================================================================

Link to news story: https://www.sciencedaily.com/releases/2022/04/220425121059.htm

--- up 8 weeks, 10 hours, 51 minutes
* Origin: -=> Castle Rock BBS <=- Now Husky HPT Powered! (1:317/3)