New technology solves mystery of respiration in Tetrahymena

Date:
March 31, 2022
Source:
University of California - Davis
Summary:
Tetrahymena, a tiny single celled-organism, turns out to be hiding
a surprising secret: it's doing respiration -- using oxygen to
generate cellular energy -- differently from other organisms such
as plants, animals or yeasts. The discovery highlights the power
of new techniques in structural biology and reveals gaps in our
knowledge of a major branch of the tree of life.



FULL STORY ========================================================================== Tetrahymena, a tiny single celled-organism, turns out to be hiding a
surprising secret: it's doing respiration -- using oxygen to generate
cellular energy - - differently from other organisms such as plants,
animals or yeasts. The discovery, published March 31 in Science,
highlights the power of new techniques in structural biology and reveals
gaps in our knowledge of a major branch of the tree of life.


==========================================================================
"We thought we knew about respiration from studying other organisms,
but this shows us how much we still don't know," said Maria Maldonado,
a postdoctoral researcher in the Department of Molecular and Cellular
Biology at the University of California, Davis and co-first author on
the paper.

Tetrahymena is a genus of free-living, single-celled organisms usually
found quietly swimming around ponds by beating their coat of tiny hairs,
or cilia.

Like us, they are eukaryotes, with their genetic material in a
nucleus. They belong to a large and diverse group of organisms called
the SAR supergroup.

With a few exceptions, such as the malaria parasite Plasmodium, the SAR supergroup is little studied.

"It's a huge proportion of the biosphere, but we don't think about them
much," Maldonado said.

Like all other eukaryotes -- and some bacteria -- Tetrahymena consume
oxygen to generate energy through respiration, said James Letts, assistant professor of molecular and cellular biology in the UC Davis College of Biological Sciences.

Oxygen comes in at the end of the series of chemical reactions involved in respiration. Electrons are passed through a chain of proteins located in structures called cristae in the inner membrane of the mitochondrion. This drives formation of water from oxygen and hydrogen atoms, pumping
protons across the membrane, which in turn drives formation of the ATP,
a store of chemical energy for the cell. This electron transport chain
is fundamental to oxygen-based respiration in humans and other eukaryotes.



==========================================================================
New approaches in structural biology There were clues that there is
something different about the electron transport chain in Tetrahymena,
Letts said. In the 1970s and 80s, scientists discovered that its electron-carrying protein -- cytochrome c -- and oxygen consuming enzyme
at the end of the chain -- terminal oxidase -- function differently than
those in plants and animals. Until now, it wasn't clear exactly how
or why these enzymes differed in Tetrahymena when they were conserved
across other studied eukaryotes.

Maldonado, Letts and co-first author Long Zhou used new approaches
in structural biology to uncover the Tetrahymena electron transport
chain. These included a cryo-electron microscopy structural proteomics
approach -- working out the structures of large number of proteins in
a mixed sample at the same time.

Cryo-electron microscopy freezes samples to extremely low temperatures, creating images at almost atomic resolution. Instead of imaging a
single, purified protein, the team worked with mixed samples isolated
from mitochondrial membranes and then taught an algorithm to recognize
related structures.

In this way, they were able to scan through hundreds of thousands of
protein images and identify the structures of 277 proteins in three
large assemblies, representing the Tetrahymena electron transport chain
at near atomic resolution. Some of these proteins have no matching gene
in the known Tetrahymena genome database -- showing that there must be
gaps in the available reference genome.

By revealing the gaps in our knowledge of a fairly common organism, the
work shows our blind spots with respect to biodiversity, Letts said. It
also shows the potential of these new methods in structural biology as
a discovery tool, he said.

Part of the work was conducted with cryo-electron microscopes at the BioEM
core facility at the UC Davis College of Biological Sciences. Additional authors on the paper are Abhilash Padavannil and Fei Guo, both at UC
Davis. Zhou is now at Zhejiang University School of Medicine, Hangzhou,
China. The work was supported by the NIH.


========================================================================== Story Source: Materials provided by
University_of_California_-_Davis. Original written by Andy Fell. Note:
Content may be edited for style and length.


========================================================================== Journal Reference:
1. Long Zhou, Mari'a Maldonado, Abhilash Padavannil, Fei Guo, James A.

Letts. Structures of Tetrahymena 's respiratory chain reveal
the diversity of eukaryotic core metabolism. Science, 2022; DOI:
10.1126/ science.abn7747 ==========================================================================

Link to news story: https://www.sciencedaily.com/releases/2022/03/220331151450.htm

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