Membraneless organelles: From liquid to solid to drive development
Date:
March 24, 2022
Source:
European Molecular Biology Laboratory
Summary:
Researchers show that transition from liquid to solid is important
for the function of membraneless organelles.
FULL STORY ==========================================================================
The term 'phase transition' might initially conjure up images of ice
melting or water vapour condensing on a cold glass. In biology, phase transition plays a role in processes such as lipid bilayer formation
or the spontaneous de-mixing of protein droplets. In a recent paper
published in Cell, the Ephrussi and Mahamid groups at EMBL Heidelberg
have now shown how phase transitions in protein-RNA droplets can influence their biological function.
==========================================================================
In order to regulate the many cellular functions within an organism, biochemical processes within individual cells must be precisely regulated
in time and space. While organelles like the nucleus or the endoplasmic reticulum are enclosed by membranes and thereby physically separate
certain reactions and processes from others, the cellular space also
contains a different class of organelles without membranes, called
condensates. Like their membrane-bound counterparts, condensates control specific functions within a cell.
In their latest study, the EMBL scientists focused on one specific
mRNA, oskar, and its role in embryo development in the model organism Drosophila melanogaster (fruit fly). In the developing fruit fly egg,
oskar mRNA must localise to a specific position within the cell to lay
the foundation for the development of the future embryo. oskar mRNA is
found in ribonucleoprotein (RNP) granules that contain proteins bound to
the RNA. These are an example of membraneless condensates. What the EMBL researchers were now able to show is that these granules have solid-like properties in the developing fruit fly egg.
"Condensates are typically thought of as liquids. But we found that
a solid state of oskar RNP granules is crucial for localisation and
function of oskar mRNA," explained Mainak Bose, postdoc in the Ephrussi
and Mahamid groups, and first author of the study. "When we genetically engineered the granules in Drosophila oocytes to be liquid-like,
it resulted in a multitude of defects in the developing embryos."
These findings demonstrate the importance of the physical properties of condensates for their physiological functions, something that was until
now believed to be governed by their biochemical properties alone. "Our
work highlights how interactions and properties at the molecular level
govern the biophysical properties and functions of condensates on the
cellular and even organismal scale," concluded Bose.
========================================================================== Story Source: Materials provided by
European_Molecular_Biology_Laboratory. Note: Content may be edited for
style and length.
========================================================================== Journal Reference:
1. Mainak Bose, Marko Lampe, Julia Mahamid, Anne
Ephrussi. Liquid-to-solid
phase transition of oskar ribonucleoprotein granules is essential
for their function in Drosophila embryonic development. Cell,
2022; DOI: 10.1016/j.cell.2022.02.022 ==========================================================================
Link to news story:
https://www.sciencedaily.com/releases/2022/03/220324104552.htm
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