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    From ScienceDaily@1:317/3 to All on Mon Mar 21 22:30:46 2022
    supplements
    Terahertz light creates twisting vibrations in biomolecules such as
    proteins, confirming whether their compositions and structures are safe and effective.

    Date:
    March 21, 2022
    Source:
    University of Michigan
    Summary:
    It's not easy to be sure that drugs and supplements with twisted --
    or chiral -- structures are turning in the correct direction. Now,
    twirling infrared light can probe both the structures of molecular
    crystals and their twists.



    FULL STORY ==========================================================================
    It's not easy to be sure that drugs and supplements with twisted -- or
    chiral - - structures are turning in the correct direction. Now, twirling infrared light can probe both the structures of molecular crystals and
    their twists, research led by the University of Michigan has shown.


    ==========================================================================
    The researchers hope that the technique could also help diagnose harmful accumulations of twisted molecules in the body, including bladder stones, insulin fibrils and amyloid aggregations such as the plaques that appear
    in Alzheimer's disease.

    In a world of curled molecules, biology often favors right- or left-handed versions. Walking along the supplement aisle, you might notice that some
    have an L or D in front of the names. L and D denote the direction in
    which the molecule twists, clockwise or counterclockwise -- the human
    body typically only uses one version. Molecules with the wrong twist
    can be nuisance fillers or cause side effects that can be unpleasant
    or dangerous. But quality control for twisted molecules is tough,
    and monitoring the chiral structures of drugs and supplements kept in
    storage isn't usually done.

    "The methods most commonly used at pharmaceutical companies are very
    sensitive to impurities, but measuring chirality is expensive," said
    Wonjin Choi, a research fellow in chemical engineering at U-M and first
    author of the paper in Nature Photonics.

    The new method can quickly recognize wrong twists and wrong chemical
    structures in packaged drugs using terahertz radiation, a portion of the infrared part of the spectrum. It was developed by an international team, including researchers at the Federal University of Sa~o Carlos, Brazil; Brazilian Biorenewables National Laboratory; University of Notre Dame;
    and Michigan State University.

    "Biomolecules support twisting, long-range vibrations also known as
    chiral phonons. These vibrations are very sensitive to the structure of molecules and their nanoscale assemblies, creating the fingerprint of a particular chiral structure," said Nicholas Kotov, the Irving Langmuir Distinguished University Professor of Chemical Sciences and Engineering
    at U-M and co-corresponding author.



    ==========================================================================
    The team was able to measure these phonons in the spectra of twisted
    terahertz light that passed through tested materials. One of these, L-carnosine, is currently used as a nutritional supplement.

    "If the twist of the molecule is wrong, if the twist in the way the
    molecules pack together is not right, or if different materials were
    mixed in, all of that could be inferred from the spectra," Kotov said.

    John Kruger, professor of veterinary medicine at Michigan State University
    and co-author of the paper, provided bladder stones from dogs, and the
    team discovered their chiral signature. The team hopes that the findings
    could help enable rapid diagnostics for pets and perhaps later humans. In addition, they studied insulin as it grew into nanofibers that make
    it inactive. If the terahertz light technology can be adapted for home healthcare, it could verify the quality of insulin.

    The team also explored how light can influence structures, rather than
    just measure them. Calculations carried out by Andre' Farias de Moura, professor of chemistry at the Federal University of Sa~o Carlos and co-corresponding author, show that multiple biomolecules vigorously
    twist and vibrate when terahertz light generates chiral phonons.

    "We foresee new roads ahead -- for instance using terahertz waves with
    tailored polarization to manipulate large molecular assemblies. It might replace microwaves in many synthesis applications in which the handedness
    of the molecules matters," said de Moura.

    Based on de Moura's calculations, Kotov and Choi believe that the
    twisting vibrations of chiral phonons caused by terahertz light
    may make disease-causing nanofibers more vulnerable to medical
    interventions. Future work will explore whether that interaction can be
    used to break them up.

    This work was supported by the U.S. Department of Defense, Office of
    Naval Research, Defense Advanced Research Projects Agency and National
    Science Foundation; Brazilian funding agencies CAPES and FAPESP; Japanese Society for the Promotion of Science and Yoshida Foundation; and U-M.

    The materials were studied at the Michigan Center for Materials Characterization. Kotov is also the Joseph B. and Florence V. Cejka
    Professor of Engineering and professor of chemical engineering, materials science and engineering, and macromolecular science and engineering.


    ========================================================================== Story Source: Materials provided by University_of_Michigan. Note:
    Content may be edited for style and length.


    ========================================================================== Journal Reference:
    1. Choi, W.J., Yano, K., Cha, M. et al. Chiral phonons in microcrystals
    and
    nanofibrils of biomolecules. Nat. Photon, 2022 DOI:
    10.1038/s41566-022- 00969-1 ==========================================================================

    Link to news story: https://www.sciencedaily.com/releases/2022/03/220321132157.htm

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